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8.51029

Sigma-Aldrich

N-Biotinyl-NH-(PEG)₂-COOH . DIPEA (20 atoms)

Novabiochem®

Synonym(s):

N-Biotinyl-NH-(PEG)₂-COOH . DIPEA (20 atoms), O-(N-Biotinyl-3-aminopropyl)-O′-(N-glutaryl-3-aminopropyl)-diethyleneglycol · DIPEA

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About This Item

Empirical Formula (Hill Notation):
C25H44N4O8S · xC8H19N
CAS Number:
1205744-09-7
Molecular Weight:
560.70
UNSPSC Code:
12352106
NACRES:
NA.22

Quality Level

200

product line

Novabiochem®

Assay

≥95.0% (HPLC)
≥98% (TLC)

form

powder

reaction suitability

reaction type: Biotinylations

manufacturer/tradename

Novabiochem®

mp

131-136 °C

application(s)

peptide synthesis

storage temp.

2-8°C

Related Categories

General description

The use of this novel biotin derivative incorporates a hydrophilic 20 atom PEG spacer between the peptide and biotin. This not only greatly improves the solubility of the resultant peptide, but reduces steric hindrance between the peptide and biotin, leading to better avidin binding and higher biological activity. The biotin-PEG derivative can be easily incorporated using any standard coupling method as the final step in SPPS, immediately prior to cleavage of the peptide from the resin.

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Linkage

Replaces: 01-63-0133

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(CMA2)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (50 mg in 1 ml NMP): clearly soluble

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Articles

Biotinylation Reagents

Biotin-labelled peptides find applications in immunology and histochemistry for affinity purification and receptor localization.

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