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8.51025

Sigma-Aldrich

5-Carboxyfluorescein

Novabiochem®

Synonym(s):

5-Carboxyfluorescein, 5-FAM

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About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
76823-03-5
Molecular Weight:
376.32
MDL number:
MFCD00036874
UNSPSC Code:
12352005
NACRES:
NA.22

Quality Level

200

product line

Novabiochem®

Assay

≥98% (TLC)
≥99.0% (HPLC)

form

powder

reaction suitability

reagent type: fluorescent-labelling reagent

manufacturer/tradename

Novabiochem®

application(s)

peptide synthesis

storage temp.

2-8°C

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

Related Categories

General description

This high purity fluorescent dye (λex494 nm, λem 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment [1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Application

5-Carboxyfluorescein Novabiochem®. CAS 76823-03-5, molar mass 376.32 g/mol.

Linkage

Replaces: 01-63-0112

Analysis Note

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Articles

Peptide Labeling

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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