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Sigma-Aldrich

Probumin® Bovine Serum Albumin Life Science Grade

≥98%, USA origin, ≤50 μg/g IgG, cell culture| mammalian: suitable, enzyme immunoassay: suitable, Serology: suitable

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Synonym(s):
Albumin, BSA, Bovine Serum, Bovine Serum Albumin, Fraction V
CAS Number:
9048-46-8
UNSPSC Code:
41141801

biological source

bovine serum

Quality Level

300

Assay

≥98% (purity)

form

lyophilized powder

shelf life

48 mo.

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

pkg of 5 kg

manufacturer/tradename

Probumin®

origin

USA origin

technique(s)

cell culture | mammalian: suitable using
enzyme immunoassay: suitable

dilution

(Serology)

impurities

≤50 μg/g IgG

loss

≤5%

pH

6.8-7.2

solubility

water: soluble 40 mg/mL

cation traces

heavy metals: ≤20 ppm

foreign activity

Tested for Mycoplasma
Tested for Protease

storage temp.

2-8°C

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General description

A high purity, low endotoxin and low IgG BSA powder preparation with near native albumin characteristics. This BSA is first isolated by a proprietary heat-shock/ organic solvent process and further purified by non solvent based methodologies to reduce endotoxins to very low levels resulting in a highly monomeric bovine albumin that retains many of the binding, transfer, and physical properties of native albumin. Purity is = 98%; typical purity is 100%. This BSA may be used for molecular biology applications.

Legal Information

Probumin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Probumin® BSA grades are distinguished by differences in production. The use of terms such as ‘Diagnostic Grade′ or ‘Vaccine Grade′ in product names solely indicates that said products are commonly used by customers in those applications. We have not qualified every single product in every conceivable application. Users should qualify Probumin® BSA grades using the specific reagents, conditions and constraints of their assay.

For further manufacturing use only. Not for human or animal consumption.

WGK

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

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Articles

Probumin® Bovine Serum Albumin (BSA) Powders & Solutions - Frequently Asked Questions

Probumin® BSA is a commonly used bovine serum albumin for buffers, non-specific blocking, protein stabilization and other diagnostic applications. Given the diverse uses of BSA, here are some frequently asked questions and answers about the molecule, formats, methods, and applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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