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820472

Sigma-Aldrich

Probumin® Bovine Serum Albumin Biotech Grade

≥98%, USA origin, Tested for IgG, cell culture | mammalian, Binding, transport studies

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Synonym(s):
Albumin, BSA, Bovine Serum, Bovine Serum Albumin, Fraction V
CAS Number:
9048-46-8
UNSPSC Code:
41141801

biological source

bovine serum

Quality Level

300

Assay

≥98% (purity)

form

lyophilized powder

shelf life

48 mo.

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

pkg of 10 g

manufacturer/tradename

Probumin®

origin

USA origin

Esterified fatty acids content

0-1.0 (mg/g)

Total lipids content

0-2.0 (mg/g)

free fatty acid content   content

0-0.2 (mg/g)

technique(s)

cell culture | mammalian: suitable

dilution

(Binding, transport studies)

impurities

Tested for IgG

loss

≤5%

pH

6.8-7.2

anion traces

chloride (Cl-): 0-6.0 mg/g

cation traces

Na: 0-10 mg/g

foreign activity

Endotoxin 0 - 2.0 EU/mg
Tested for mycoplasma

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General description

Highly purified BSA powder manufactured by a proprietary heat-shock fractionation process, followed by treatment to lower fatty acids and lipids. This albumin contains one of the lowest levels of total lipids, fatty acids and free fatty acids of any commercially available albumin. Endotoxin and IgG levels are also very low to absent. This bovine albumin product may be used for cell culture applications as well as a stabilizing agent and for blocking of nonspecific binding. Purity is = 98%; typical purity is 100%.

Storage and Stability

Storage temperature <-10 °C

Legal Information

Probumin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Probumin® BSA grades are distinguished by differences in production. The use of terms such as ‘Diagnostic Grade′ or ‘Vaccine Grade′ in product names solely indicates that said products are commonly used by customers in those applications. We have not qualified every single product in every conceivable application. Users should qualify Probumin® BSA grades using the specific reagents, conditions and constraints of their assay.

For further manufacturing use only. Not for human or animal consumption.

WGK

WGK 3

Regulatory Information

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Articles

Probumin® Bovine Serum Albumin (BSA) Powders & Solutions - Frequently Asked Questions

Probumin® BSA is a commonly used bovine serum albumin for buffers, non-specific blocking, protein stabilization and other diagnostic applications. Given the diverse uses of BSA, here are some frequently asked questions and answers about the molecule, formats, methods, and applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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