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71491

Sigma-Aldrich

Overnight Express Instant TB Medium - Novagen

Synonym(s):

autoinduction media 

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20 μG
CN¥2,225.48

CN¥2,225.48


Estimated to ship onApril 14, 2025Details


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20 μG
CN¥2,225.48

About This Item

UNSPSC Code:
41106213
NACRES:
NA.54

CN¥2,225.48


Estimated to ship onApril 14, 2025Details


Request a Bulk Order

form

solid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

microbiological culture: suitable

application(s)

microbiology

shipped in

ambient

storage temp.

10-30°C

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This Item
SAB4200782T9030P9318
biological source

rat

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

mouse

species reactivity

human

species reactivity

vertebrates, chicken

species reactivity

marsupial (Ptoorous tridactylis PtK2 cell line), rat

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

clone

MTn-12, monoclonal

clone

BC-24, monoclonal

clone

T11, monoclonal

clone

7A8, monoclonal

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

General description

Overnight Express Instant TB Medium is a complete autoinduction media in granulated terrific broth formulation. It is used in routine recombinant protein expression applications. Overnight Express Instant TB Medium is ideal for producing recombinant protein without monitoring cell growth or inducer (Isopropyl ß-D-1-thiogalactopyranoside(IPTG)) addition through lac operon-controlled Escherichia coli expression systems.

Application

Overnight Express Instant TB Medium – Novagen has been used:
  • to culture recombinant Escherichia coli colonies for protein expression.[1][2][3]
  • as an auto-induction media to study the effect of glucose and lactose additives on recombinant ovine angiotensinogen (oANG) production[4]
  • as a medium for hamster recombinant PrP (rPrP) protein expression[5]

Features and Benefits

  • Autoinduction media: simplifies protocol by eliminating the monitoring and induction steps
  • Rich culture media: high cell densities and protein expression level
  • Granulated format: rapid and uniform dissolution in water minimize the chance of inhaling hazardous powdered ingredients
  • Singe use EasyPak packaging: Minimal sample handling significantly reduces the chance of contamination.
  • Convenient, no need to spend extra time on weighting out different ingredients

Warning

Toxicity: Standard Handling (A)

Preparation Note

The EasyPak aluminum foil pouch contains enough granulated medium to prepare 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and bring it to a boil in a microwave oven for 2 minutes.

Legal Information

Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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    Nariyasu Tahara et al.
    Protein and peptide letters, 28(10), 1180-1190 (2021-08-07)
    Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems. Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates. The differences in culture substrates sometimes lead
    Jun-Jie Liu et al.
    Journal of immunoassay & immunochemistry, 33(4), 352-368 (2012-09-12)
    RGD peptide (Arg-Gly-Asp tripeptide) binds to integrin αVβ(3) and αVβ(5), which is selectively expressed in tumor neovasculature and on the surface of some tumor cells. Some studies showed that coupling the RGD peptides to anticancer drugs yielded compounds with increased
    Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution.
    Mancini, et al.
    Protein Science, 16, 2294-2300 (2018)
    Sarah Weisman et al.
    Biomaterials, 31(9), 2695-2700 (2009-12-29)
    Transgenic production of silkworm and spider silks as biomaterials has posed intrinsic problems due to the large size and repetitive nature of the silk proteins. In contrast the silk of honeybees (Apis mellifera) is composed of a family of four
    Kang Xiao et al.
    International journal of molecular medicine, 42(6), 3231-3237 (2018-09-18)
    In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of

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