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form
solid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
microbiological culture: suitable
application(s)
microbiology
shipped in
ambient
storage temp.
10-30°C
Related Categories
General description
Overnight Express™ Instant TB Medium is a complete autoinduction media in granulated terrific broth formulation. It is used in routine recombinant protein expression applications. Overnight Express Instant TB Medium is ideal for producing recombinant protein without monitoring cell growth or inducer (Isopropyl ß-D-1-thiogalactopyranoside(IPTG)) addition through lac operon-controlled Escherichia coli expression systems.
Application
Overnight Express Instant TB Medium – Novagen has been used:
- to culture recombinant Escherichia coli colonies for protein expression.
- as an auto-induction media to study the effect of glucose and lactose additives on recombinant ovine angiotensinogen (oANG) production
- as a medium for hamster recombinant PrP (rPrP) protein expression
Features and Benefits
- Autoinduction media: simplifies protocol by eliminating the monitoring and induction steps
- Rich culture media: high cell densities and protein expression level
- Granulated format: rapid and uniform dissolution in water minimize the chance of inhaling hazardous powdered ingredients
- Singe use EasyPak packaging: Minimal sample handling significantly reduces the chance of contamination.
- Convenient, no need to spend extra time on weighting out different ingredients
Warning
Toxicity: Standard Handling (A)
Preparation Note
The EasyPak aluminum foil pouch contains enough granulated medium to prepare 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and bring it to a boil in a microwave oven for 2 minutes.
Legal Information
Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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