71340-M
pCDFDuet-1 DNA - Novagen
Synonym(s):
Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
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About This Item
UNSPSC Code:
41106602
NACRES:
NA.51
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
Promoter
Promoter name: T7
selection
spectinomycin
storage temp.
−70°C
General description
pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
Application
pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.
Warning
Toxicity: Standard Handling (A)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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