rLysozyme Solution

rLysozyme Solution contains a highlypurified and stabilized recombinant lysozymethat can be used for lysis of Gram-negativebacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction withBugBuster, BugBuster HT, and PopCultureReagents. In the absence of protein extractionreagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON X-100, pH 7.5. rLysozyme Solution is stable at –20°C.

Toxicity: Standard Handling (A)

One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany