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NovaBlue(DE3) Competent Cells - Novagen

Name: NovaBlue(DE3) Competent Cells - Novagen
Brand: MM

Escherichia coli, rod shaped

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About This Item

UNSPSC Code:

41106202

NACRES:

NA.81

product name

NovaBlue(DE3) Competent Cells - Novagen, NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, DE3 lysogen cannot be used for the blue/white screening of recombinant plasmids

biological source

Escherichia coli

Quality Level

100

manufacturer/tradename

Novagen^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

transformation efficiency: >1×10⁸ cfu/μg

shipped in

dry ice

storage temp.

−70°C

Related Categories

Molecular Cloning & Protein Expression

General description

NovaBlue(DE3) Competent Cells is a K-12 strain with high transformation efficiency and recA endA mutations. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacI^qrepressor encoded by the F episome.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlNovaBlue(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

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Non-canonical Activation of the DNA Sensing Adaptor STING by ATM and IFI16 Mediates NF-κB Signaling after Nuclear DNA Damage.

Gillian Dunphy et al.

Molecular cell, 71(5), 745-760 (2018-09-08)

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