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688225

Sigma-Aldrich

Caspase-1 Substrate VI, Fluorogenic

Fluorogenic caspase-1 substrate.

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Synonym(s):
Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
Empirical Formula (Hill Notation):
C33H36F3N5O10
CAS Number:
219137-85-6
Molecular Weight:
719.66
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white to off-white

solubility

DMSO: 5 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

shipped in

ambient

storage temp.

−20°C

InChI

1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1

InChI key

GWKJISJKNKIJNP-DEQDFGQZSA-N

Related Categories

General description

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.
Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Fluorogenic caspase-1 substrate
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Tyr-Val-Ala-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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