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Key Documents

650366-M

Sigma-Aldrich

Tryptase, Human Lung

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352202
NACRES:
NA.77

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form

liquid

Quality Level

specific activity

≥50 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

storage temp.

−20°C

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This Item
PHR1107G8415G1251
technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

microbiological culture: suitable

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

application(s)

cleaning products
cosmetics
food and beverages
personal care
pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

-

format

neat

format

neat

format

-

format

-

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

grade

certified reference material, TraceCERT®

grade

certified reference material, pharmaceutical secondary standard

grade

-

grade

-

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

General description

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Reported to upregulate neoangiogenesis in carcinogenesis of the uterine cortex. Tryptase is also an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

Warning

Toxicity: Irritant (B)

Unit Definition

One unit is defined as the amount of enzyme that gives a change of 1 absorbance per min at 410 nm using N-benzoyl-Phe-Val-Arg-pNA as a substrate in 30 min at 25°C.

Physical form

In 1 M NaCl, 50 mM NaOAc, 50 µM heparin, pH 5.0.

Preparation Note

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Benitez-Bribiesca, L., et al. 2001. J. Histochem. Cytochem.49, 1061.
He, S., et al. 1998. J. Pharmacol. Exp. Ther. 286, 289.
Molino, M., et al. 1997. J. Biol. Chem. 272, 4043.
Schwartz, L.B., and Bradford, T.R. 1986. J. Biol. Chem. 261, 7372.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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