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65035-M

Sigma-Aldrich

Trypsin Inhibitor, Soybean

Synonym(s):

Trypsin Inhibitor, Soybean

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.77

form

lyophilized solid

Quality Level

specific activity

≥4000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

off-white

solubility

67 mM potassium phosphate buffer, pH 7.6: 5 mg/mL

storage temp.

−20°C

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General description

A reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, pH-dependent manner (Kd = 500 nM at pH 7.8).
Reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, reversible and pH-dependent manner (Kd = 0.5 nM at pH 7.8). Also inhibits, to a lesser degree, chymotrypsin, kallikrein, plasmin, and other serine proteases.

Biochem/physiol Actions

Kd = 500 nM at pH 7.8 against trypsin
Primary Target
trypsin

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of protein that will inhibit 1 unit of trypsin activity using BAEE as a substrate at 25°C, pH 7.6.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C.

Other Notes

Lilly, C.M., et al. 1993. J. Clin. Invest.91, 235.
Uchino, T., et al. 1993. J. Biol. Chem.268, 527.
Birk, Y. 1976. Methods Enyzmol.45, 700.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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