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648317

Millipore

Tris, Hydrochloride, Molecular Biology Grade

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

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Synonym(s):
Trizma® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:
MFCD00012590
UNSPSC Code:
12161705
NACRES:
NA.25

grade

Molecular Biology

Quality Level

100

Assay

≥99% (titration)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤0.0002%

absorption

≤0.05 at 260 nm
≤0.05 at 280 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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General description

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Warning

Toxicity: Standard Handling (A)

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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