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Merck
CN

648313

TRIS Hydrochloride

ULTROL® Grade, ≥99% (titration), useful in pH range 7.0-9.0

Synonym(s):

Trizma® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
NACRES:
NA.25
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
3675235
MDL number:
MFCD00012590

Key Documents

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Product Name

TRIS Hydrochloride, ULTROL® Grade, ≥99% (titration), useful in pH range 7.0-9.0

SMILES string

Cl.NC(CO)(CO)CO

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

description

Merck USA index - 14, 9772

assay

≥99% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

solubility

water: 500 mg/mL

cation traces

heavy metals: ≤5 ppm

shipped in

ambient

storage temp.

15-25°C

Quality Level

100

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Legal Information

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Application


  • Effect of Organic Solvents on the Activity, Stability and Secondary Structure of asclepain cI, Using FTIR and Molecular Dynamics Simulations.: This study uses Tris hydrochloride as a buffer to assess the stability and structural changes in the asclepain cI enzyme, providing insights into enzyme applications in industrial and medical biotechnology (Origone et al., 2024).

  • Microencapsulation of Omeprazole by Lactobacillus acidophilus ATCC 4356 Surface Layer Protein and Evaluation of its Stability in Acidic Condition.: Research demonstrates the use of Tris hydrochloride buffer in stabilizing microencapsulated omeprazole, aimed at enhancing drug delivery in acidic environments (Mobarak Qamsari et al., 2020).

Disclaimer

Toxicity: Standard Handling (A)

General description

Absorbance (1.0 M, H2O, 260 nm):≤0.1.
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used as a biological buffer (absorbance: <0.1 at 260 nm, 1.0 M, H2O). A 1.0 M solution in H2O has a pH range of 3.5-5.0 and a pKa of 8.3 at 20°C.

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Xiulan Shao et al.
Oncology letters, 21(6), 431-431 (2021-04-20)
Ovarian cancer is one of the leading causes of cancer-related death among women worldwide and accounts for 4% of all cancer cases in female patients. To date, ovarian cancer has the poorest prognosis among all types of gynecological cancer; thus

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