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Merck
CN

6.18729

Trifluoroacetic acid

suitable for chromatography

Synonym(s):

Trifluoroacetic acid solution, TFA

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About This Item

Linear Formula:
CF3COOH
CAS Number:
Molecular Weight:
114.02
UNSPSC Code:
12352106
Beilstein/REAXYS Number:
5446934
MDL number:
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Product Name

Trifluoroacetic acid, suitable for chromatography

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

SMILES string

OC(=O)C(F)(F)F

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

suitability

suitable for chromatography

Quality Level

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Application

Trifluoroacetic acid can be used as:
  • Ion-pairing reagent in reversed-phase liquid chromatography (RPLC) for peptide separation.
  • An acidic additive in the mobile phase for chiral supercritical fluid chromatography.

General description

Trifluoroaceticacid (TFA) is used as an additive in HPLC and LC-MS analyses to improve theseparation of basic compounds.

pictograms

CorrosionExclamation mark

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

>212.0 °F - Pensky-Martens closed cup

flash_point_c

> 100 °C - Pensky-Martens closed cup

Regulatory Information

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Certificates of Analysis (COA)

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Utility of retention prediction model for investigation of peptide separation selectivity in reversed-phase liquid chromatography: impact of concentration of trifluoroacetic acid, column temperature, gradient slope and type of stationary phase
Analytical Chemistry, 82(1), 265-275 (2010)
Combined use of isopropylamine and trifluoroacetic acid in methanol-containing mobile phases for chiral supercritical fluid chromatography
De Klerck K, et al.
Journal of Chromatography A, 1234, 72-79 (2012)

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