Tissue Plasminogen Activator, Human

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca²⁺ antagonist may increase levels of tPA.

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acids. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration.

Toxicity: Standard Handling (A)

Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween^®-80 Detergent.

Prepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Reconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).

Kim, Y.H., et al. 1999. Science284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC