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580560

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TCEP, Hydrochloride

A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.

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Synonym(s):
TCEP, Hydrochloride, tris(2-Carboxyethyl)phosphine, HCl
Empirical Formula (Hill Notation):
C9H15O6P · xHCl
CAS Number:
51805-45-9
Molecular Weight:
250.19 (free base basis)
MDL number:
MFCD00145469
UNSPSC Code:
12352128
NACRES:
NA.25

Quality Level

100

Assay

≥98% (elemental analysis)

form

crystalline solid

reaction suitability

reagent type: reductant

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white

solubility

water: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C9H15O6P/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15/h1-6H2,(H,10,11)(H,12,13)(H,14,15)/p-3

InChI key

PZBFGYYEXUXCOF-UHFFFAOYSA-K

Related Categories

General description

A water-soluble and odorless disulfide reducing agent which is more stable and effective than DTT. TCEP reduces even the most stable water-soluble alkyl disulfides at pH 4.5 within 5 min. TCEP is also capable of functioning as a reducing agent even in pH range of 1.5-8.5. Is more resistant to air-oxidation as compared to DTT.

Application


  • Peptide and Protein Desulfurization with Diboron Reagents.: This study investigates the use of TCEP hydrochloride in peptide and protein desulfurization, demonstrating its effectiveness in facilitating diboron-mediated reactions. (Jing & Walczak, 2024).

  • A Study on Thiol-Michael Addition to Semi-Synthetic Elastin-Hyaluronan Material for Electrospun Scaffolds.: TCEP hydrochloride is utilized to enhance the thiol-Michael addition reaction in the development of electrospun scaffolds, contributing to advanced biomaterials research. (Laezza et al., 2024).

  • Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis.: This paper presents an optimized protocol for RNA isolation from bovine sperm, highlighting the role of TCEP hydrochloride in improving sperm head lysis efficiency. (Tiwari et al., 2024).

  • Target Recognition-Triggered Peroxidase-Mimicking Activity Depression in Homochiral Nanochannels for Identifying Cystine Enantiomers.: The study uses TCEP hydrochloride in a novel peroxidase-mimicking system to differentiate cystine enantiomers, showcasing its application in chiral analysis. (Xu et al., 2023).

  • In situ digestion of alcohol-fixed cells for quantitative proteomics.: TCEP hydrochloride is applied in the in situ digestion of fixed cells to enhance the accuracy of quantitative proteomics analyses. (Hatano et al., 2023).

Packaging

Packaged under inert gas

Warning

Toxicity: Corrosive (L)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

WGK

WGK 1

Certificates of Analysis (COA)

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