574793
TE Buffer, 100X, Molecular Biology Grade
A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.
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Synonym(s):
Tris-EDTA Buffer
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
grade
Molecular Biology
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
foreign activity
DNase, RNase, protease, none detected
shipped in
ambient
storage temp.
15-25°C
General description
A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.
Tris-ethylenediaminetetraacetic (TE) buffer is a common buffer used in molecular biology to maintain the integrity of nucleic acid during handling and storage.
Application
TE Buffer, 100X, Molecular Biology Grade – Calbiochem has been used to dilute DNA stock solutions. It has also been used to dilute thymidylate synthase (thyA) culture for colony polymerase chain reaction (PCR).
Warning
Toxicity: Irritant (B)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
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Jason W Johnston et al.
Plant physiology and biochemistry : PPB, 43(9), 844-853 (2005-11-18)
HPLC analysis of nucleosides is important for determining total DNA methylation in plants and can be used to help characterise epigenetic changes during stress, growth and development. This is of particular interest for in vitro plant cultures as they are
Ping Song et al.
Nature biomedical engineering, 5(7), 690-701 (2021-05-05)
DNA sequence variants with allele fractions below 1% are difficult to detect and quantify by sequencing owing to intrinsic errors in sequencing-by-synthesis methods. Although molecular-identifier barcodes can detect mutations with a variant-allele frequency (VAF) as low as 0.1% using next-generation
Victor Chen et al.
Applied and environmental microbiology, 87(18), e0084421-e0084421 (2021-07-08)
Enterococcus faecium is a ubiquitous Gram-positive bacterium that has been recovered from the environment, food, and microbiota of mammals. Commensal strains of E. faecium can confer beneficial effects on host physiology and immunity, but antibiotic usage has afforded antibiotic-resistant and
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