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567707

Sigma-Aldrich

Sphingomyelinase, Staphylococcus aureus

Synonym(s):

Sphingomyelinase, Staphylococcus aureus

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54

biological source

Staphylococcus aureus

Quality Level

form

liquid

specific activity

≥100 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

General description

Native sphingomyelinase from Staphylococcus species. May be applied exogenously to initiate the sphingomyelin signaling pathway. Has very little activity on phosphatidylcholine.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of trinitrophenylaminolauryl (TNPAL)-sphingomyelin per min at 37°C, pH 7.4.

Physical form

In 250 mM phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial use, aliquot and refrigerate (4°C).

Other Notes

Galve-Roperh, I., et al. 1997. Brain Res. Mol. Brain Res.52, 90.
Jarvis, W.D., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 73.
Mathias, S., et al. 1993. Science259, 519.
Raines, M.A., et al. 1993. J. Biol. Chem.268, 14572.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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