5.59844
Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)
liquid, clone SMI-32, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
SMI-32
form
liquid
species reactivity
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
storage temp.
2-8°C
Gene Information
human ... NEFH(4744)
General description
Mouse monoclonal antibody supplied as affinity purified antibody. Recognizes the ~180-200 kDa non-phosphorylated neurofilament H protein.
Recognizes the non-phosphorylated ~180 kDa-200 kDa neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations.
This Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Neurofilament H Non-Phosphorylated.
Immunogen
Rat
homogenized hypothalami from Fischer 344 rat brain
Application
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000, see comments)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000, see comments)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS
Reconstitution
Only recognizes non-phosphorylated neurofilament H. By immunocytochemistry this antibody stains neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems, but does not stain thin axons or other cells and tissues. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehye-containing fixatives, such as Bouin′s fixative. Antibody reactivity is poor in glutaraldehyde/paraformaldehyde-fixed samples. For staining formalin-fixed, paraffin sections it is recommended that de-paraffinized tissue be autoclaved in dH₂O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min. Trypsin pre-treatment abolishes antibody binding to the epitope. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections or thick sections fixed in 4% paraformaldehyde and in cultured cells. By immunoblotting this antibody detects two bands, ~180 and ~200 kDa, which merge into a single NFH line on two-dimensional gels. Antibody should be titrated for optimal results in individual systems.
Other Notes
Trapp, B.D., et al. 1998. N. Engl. J. Med.338, 278.
King, C.E., et al. 1997. Neuroreport.8, 1663.
Campbell, M.J., et al. 1991. Brain Res.539, 133.
Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191.
Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
King, C.E., et al. 1997. Neuroreport.8, 1663.
Campbell, M.J., et al. 1991. Brain Res.539, 133.
Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191.
Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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