556881
Ribonuclease Inhibitor, Human, Recombinant, E. coli
The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
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UNSPSC Code:
12352200
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Quality Level
form
liquid
specific activity
≥20,000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
foreign activity
Endonuclease and RNase, none detected
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Biochem/physiol Actions
Cell permeable: no
Primary Target
inactivates RNase
inactivates RNase
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
Physical form
In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.
Reconstitution
Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.
Other Notes
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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