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550701-M

Sigma-Aldrich

Pyruvate Oxidase, Aerococcus viridans

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

Synonym(s):

Pyruvate Oxidase, Aerococcus viridans

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54

Quality Level

form

lyophilized solid

specific activity

≥25 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

impurities

≤0.006% GPT

color

yellow

pI 

4.0

solubility

aqueous buffer: soluble

foreign activity

GOT and lactate oxidase ≤0.002%
catalase ≤0.3%

storage temp.

−20°C

General description

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme which generates 1 µmol of H₂O₂ per min at 37°C, pH 6.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hager, L.P. and Lipmann, F. 1955. Methods Enzymol. 1, 482.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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