550701-M
Pyruvate Oxidase, Aerococcus viridans
Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.
Synonym(s):
Pyruvate Oxidase, Aerococcus viridans
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About This Item
Quality Level
form
lyophilized solid
specific activity
≥25 U/mg
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
impurities
≤0.006% GPT
color
yellow
pI
4.0
solubility
aqueous buffer: soluble
foreign activity
GOT and lactate oxidase ≤0.002%
catalase ≤0.3%
storage temp.
−20°C
General description
Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme which generates 1 µmol of H₂O₂ per min at 37°C, pH 6.5.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Hager, L.P. and Lipmann, F. 1955. Methods Enzymol. 1, 482.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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