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539576

Sigma-Aldrich

PKA, Catalytic Subunit, Bovine Heart

Synonym(s):

cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

biological source

bovine heart

Quality Level

100

Assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥20,000 units/μg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

technique(s)

activity assay: suitable

suitability

suitable for molecular biology

shipped in

wet ice

storage temp.

−70°C

General description

Research area: Cell Signaling

Native PKA catalytic subunit purified from bovine heart. Protein Kinase A (PKA) is a serine protein kinase that targets serine and threonine residues.

Biochem/physiol Actions

Protein kinases are crucial for integrating signaling networks within eukaryotic cells. It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.

Physical form

In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Corbin, J.D., et al. 1988. Methods Enzymol159, 74.
Rannels, S.R., et al 1983. Methods Enzymol99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem.66, 253.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S S Taylor et al.
Biochimica et biophysica acta, 1697(1-2), 259-269 (2004-03-17)
Protein kinases play a critical role in the integration of signaling networks in eukaryotic cells. cAMP-dependent protein kinase (PKA) serves as a prototype for this large and highly diverse enzyme family. The catalytic subunit of PKA provides the best example
Protein Kinase A Activity and Hedgehog Signaling Pathway
Vitamins and Hormones, 88.0, 273-291 (2012)
Protein kinase A
Morrison H
Journal of Separation Science, 173-178 (2021)

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