539166
Anti-19S Proteasome S2-Subunit Rabbit pAb
liquid, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Recognizes the ~100 kDa 19S proteasome S2-subunit in HeLa cells and partially purified 26S proteasome preparations. Also recognizes an ~50 kDa potential subunit degradation product.
Immunogen
Human
recombinant, human 19S proteasome S2-subunit
Application
Immunoblotting (1:1000)
Immunoprecipitation (see comments)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 1 mg/ml BSA.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells
HeLa cells
Other Notes
Also detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Kloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol.2, 179.
Yew, P.R. 2001. J. Cell Physiol.187, 1.
Chaturvedi, K., et al. 2000. Biopolymers55, 334.
Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys.383, 1.
Pickart, C.M. 2000. Trends Biochem Sci.25, 544.
Yew, P.R. 2001. J. Cell Physiol.187, 1.
Chaturvedi, K., et al. 2000. Biopolymers55, 334.
Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys.383, 1.
Pickart, C.M. 2000. Trends Biochem Sci.25, 544.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Vittorio Necchi et al.
Toxins, 11(11) (2019-11-17)
A key role in the carcinogenic action of Helicobacter pylori is played by the effector protein CagA, the first identified oncoprotein of the bacterial world. However, the present knowledge in regard to the bacterial injection of CagA into epithelial cells
David S Pitcher et al.
Biochimica et biophysica acta, 1844(12), 2222-2228 (2014-09-07)
We report that subunits of human nuclear proteasomes carry a previously unrecognised, constitutive posttranslational modification. Subunits with this modification are not visualised by SDS-PAGE, which is used in almost all denaturing protein gel electrophoresis. In contrast, CTAB-PAGE readily visualises such
Vittorio Necchi et al.
Scientific reports, 7(1), 14526-14526 (2017-11-08)
Uptake, intracellular trafficking and pathologic effects of VacA toxin from Helicobacter pylori have been widely investigated in vitro. However, no systematic analysis investigated VacA intracellular distribution and fate in H. pylori-infected human gastric epithelium in vivo, using ultrastructural immunocytochemistry that
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