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Merck
CN

5.32129

Intracellular pH Sensor, TPE-Cy

Synonym(s):

pHi Sensor

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About This Item

UNSPSC Code:
12352200

Key Documents

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assay

≥98% (HPLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

red-orange

solubility

DMSO: 100 mg/mL

Quality Level

200

General description

A cell-permeable TPE-Cy adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range.
A cell-permeable, biocompatible tetraphenylethene-cyanine (TPE-Cy) adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range. Displays a large Stokes shift and exhibits aggregation-induced emission (AIE). Following its entry into live cells, it is distributed in the cytoplasm, accumulates in organelles, and becomes fluorescent. Interacts with the lipid components of the cells and shifts its color transition point of the red-to-blue emission from extracellular pH 10 to intracellular physiological pH range. At neutral pH it provides a strong blue emission (peak at 489 nm), which is enhanced at pH 8.07. However, acidification of medium causes a reduction in the blue emission (489 nm) and induces red emission (peak at 615 nm). The ratiometric red and blue signals can serve as an indicator for local proton concentration in the cell. Does not affect cell viability up to 12.5 µM concentration.

Biochem/physiol Actions

Cell permeable: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Chen, S., et al. 2013. J. Am. Chem. Soc.135, 4926.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品
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Certificates of Analysis (COA)

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