Intracellular pH Sensor, TPE-Cy

A cell-permeable TPE-Cy adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range.

A cell-permeable, biocompatible tetraphenylethene-cyanine (TPE-Cy) adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range. Displays a large Stokes shift and exhibits aggregation-induced emission (AIE). Following its entry into live cells, it is distributed in the cytoplasm, accumulates in organelles, and becomes fluorescent. Interacts with the lipid components of the cells and shifts its color transition point of the red-to-blue emission from extracellular pH 10 to intracellular physiological pH range. At neutral pH it provides a strong blue emission (peak at 489 nm), which is enhanced at pH 8.07. However, acidification of medium causes a reduction in the blue emission (489 nm) and induces red emission (peak at 615 nm). The ratiometric red and blue signals can serve as an indicator for local proton concentration in the cell. Does not affect cell viability up to 12.5 µM concentration.

A cell-permeable, biocompatible tetraphenylethene-cyanine (TPE-Cy) adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range. Displays a large Stokes shift and exhibits aggregation-induced emission (AIE). Following its entry into live cells, it is distributed in the cytoplasm, accumulates in organelles, and becomes fluorescent. Interacts with the lipid components of the cells and shifts its color transition point of the red-to-blue emission from extracellular pH 10 to intracellular physiological pH range. At neutral pH it provides a strong blue emission (peak at 489 nm), which is enhanced at pH 8.07. However, acidification of medium causes a reduction in the blue emission (489 nm) and induces red emission (peak at 615 nm). The ratiometric red and blue signals can serve as an indicator for local proton concentration in the cell. Does not affect cell viability up to 12.5 µM concentration.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Cell permeable: yes

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Chen, S., et al. 2013. J. Am. Chem. Soc.135, 4926.

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