Plasminogen, Glu-Type, Human Plasma

Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein found in normal plasma at ~12 mg per deciliter, and is the inactive precursor of the serine protease plasmin.

Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein zymogen, which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 µM. The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls, and 5 regions of internal sequence homology, known as kringles, between Lys⁷⁷ and Arg⁵⁶⁰. These kringle regions are homologous to the kringle domains in t-PA, u-PA, and prothrombin. Native Glu-plasminogen is readily converted to Lys⁷⁷-plasminogen by plasmin hydrolysis of the Lys⁷⁶-Lys⁷⁷ peptide bond. The plasminogen is converted to plasmin by cleavage at Arg⁵⁴⁰-Val⁵⁶¹ by urokinase, streptokinase, or tissue plasminogen activator.

Toxicity: Irritant (B)

In 100 mM NaCl, 50 mM Tris-HCl, pH 7.5.

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Following initial thaw, aliquot and freeze (-20°C).

Single band by SDS-PAGE

Collen, D. 1986. In Blood Coagulation (Zwaal, R.E.A., and Hemker, H.C. eds.) Elsevier, N.Y. p. 243.
Barlow, G.H., et al. 1984. Biochemistry23, 2384.
Wohl, R.C., et al. 1982. Thromb. Res.27, 523.
Castellino, F.J., et al. 1981. Methods Enzymol.80, 365.
Robbins, K.C. 1976. Methods Enzymol.45, 257.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.