525200-M
Phospholipase D, Streptomyces chromofuscus
Synonym(s):
Phospholipase D, Streptomyces chromofuscus, PLD
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
form
lyophilized solid
Quality Level
specific activity
≥50 units/mg dry wt.
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
color
brownish
solubility
10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON™ X-100: 1 mg/mL
foreign activity
Aatalase, glucose oxidase, none detected
storage temp.
−20°C
General description
Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.
Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline; coupled with choline oxidase, it can be used for the determination of phospholipids in serum.
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.
Other Notes
Kurz, T., et al. 1993. Circ. Res. 72, 701.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Regulatory Information
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