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524742

Sigma-Aldrich

Phosphodiesterase 3A, Active, Human, Recombinant, S. frugiperda

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Synonym(s):
cAMP-specific phosphodiesterase 3A, PDE3A
UNSPSC Code:
12352203
NACRES:
NA.54

recombinant

expressed in insect cells (S. frugiperda)

Quality Level

form

liquid

specific activity

≥0.5 U/μL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Other Notes

Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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