524715
Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda
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Synonym(s):
PDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
form
liquid
Quality Level
specific activity
≥50,000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).
Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
Physical form
In sodium phosphate buffer, 20% glycerol, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Certificates of Analysis (COA)
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