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5.08838

Millipore

Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested

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Synonym(s):
Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested, (2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
Empirical Formula (Hill Notation):
C22H29N7O5 · 2HCl
CAS Number:
58-58-2
Molecular Weight:
544.43
MDL number:
MFCD00012691
UNSPSC Code:
12352101

Assay

≥98% (HPLC)

Quality Level

200

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

impurities

≤10 EU/mg Endotoxin (dry weight)

storage temp.

−20°C

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1

InChI key

MKSVFGKWZLUTTO-USYAMCSGSA-N

General description

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.
An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.

Biochem/physiol Actions

Primary Target
RNA
Reversible: yes

Warning

Toxicity: Standard Handling (A)

Physical form

Supplied at 10 mg/ml in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Other Notes

Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Kaufman, S.H., et al. 1993. Cancer Res.53, 3976.
de la Luna, S. and Ortin, J. 1992. Methods Enzymol.216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res.216, 149.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

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