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Merck
CN

492899

Nuclease, Staphylococcus aureus

Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups.

Synonym(s):

Nuclease, Staphylococcus aureus

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
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Product Name

Nuclease, Staphylococcus aureus, Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups.

biological source

Staphylococcus aureus

form

lyophilized solid

specific activity

≥15,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

color

off-white

solubility

aqueous buffer: 1 mg/mL

shipped in

ambient

storage temp.

2-8°C

Quality Level

Disclaimer

Toxicity: Standard Handling (A)

General description

Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.

Other Notes

Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.
One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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