MILLIPLEX^® Human Cell Health Magnetic Bead Panel

Cell health is affected by the careful balance of various physiological processes and signaling pathways in response to diverse stimuli including drug treatment, disease, culture conditions, and stress. For example, cells respond to different stressors by expressing stress-related proteins that may lead to the activation of survival pathways or initiation of cell death. Identifying markers of specific stressors (for example oxidative, heat shock, genotoxicity, ER stress, inflammation, et cetera), activated repair or death pathways (for example, apoptosis, autophagy, DNA repair/damage, et cetera), and affected cellular functions (mRNA synthesis, chromatin condensation, proliferation, OXPHOS, et cetera) may elucidate further details about ongoing pathological processes or pharmacological response mechanisms. Cell health panel includes all the key markers a researcher would need to analyze the health and quality of the cells.

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

MILLIPLEX^® Human Cell Health Magnetic Bead Panel is a 16-plex kit to be used for the simultaneous detection of all the following analytes in cell lysates: CHOP, Cyclin B1, Complex IV, Ki67, LC3B, HIF-1a, Polyubiquitin K48-linkage, HSP70, cleaved PARP, and GRP78 and phosphorylated eIF4B (Ser422), NF-κB (Ser536), Histone H2A.x (Ser139), Histone H3 (Ser10), p53 (Ser15), and RNA Pol II (Ser2) in cell lysates using the Luminex^® system. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

• This is an overnight assay.
• Each assay requires 25 µL per well of diluted or neat stimulated cell lysate.
• This is a qualitative assay and provides insights into the expression levels of the mentioned analytes in response to stress/treatment or condition.
• The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1000 μg/mL).
• The panel can provide mechanistic insights to researchers performing drug tox screen, studying changes in cell health due to disease/stress, or to study the expression patterns of analytes involved in various cell death and survival pathways.

This is a 16 plex fixed panel. Can be run on any of the existing Luminex xMAP platforms.

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.