48-690MAG
MILLIPLEX® T-Cell Receptor Signaling Panel
Premixed T-Cell Receptor Phosphoprotein 7-Plex Cell Signaling Panel
Synonym(s):
7-Plex Magnetic Bead Kit, Magnetic Bead Kit, T-Cell Receptor Signaling Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000
Product Name
MILLIPLEX® T-Cell Receptor Signaling Panel, Premixed T-Cell Receptor Phosphoprotein 7-Plex Cell Signaling Panel
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
packaging
1 ea of
technique(s)
multiplexing: suitable
input
cell lysate
assay range
inter-assay cv: 4-17%
intra-assay cv: 3-8%
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
Application
Research Category
Cell Signaling
Cell Signaling
This cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
Used to detect/quantify:CD3ε (pan Tyr)|CREB (S133)|ERK (Thr185/Tyr187)|LAT (pan Tyr)|Lck (pan Tyr)|Syk (pan Tyr)|ZAP-70 (pan Tyr)
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 7 analytes in cell lysate samples.Analytes Included: CD3ζ (pY142), LAT (pY191), Lck (pY394), PLCγ1 (pY783), ZAP70 (pY319), SLP-76 (pY128), ERK (pT185/pY187)Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Biochem/physiol Actions
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
General description
The Milliplex® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
This panel includes phosphorylated proteins central to T-cell receptor signaling and immune activation. CD3ζ (pY142) initiates TCR signaling upon antigen recognition. LAT (pY191) and SLP-76 (pY128) serve as scaffolding proteins for downstream signaling. Lck (pY394) and ZAP70 (pY319) are tyrosine kinases that propagate TCR signals. PLCγ1 (pY783) mediates calcium signaling, while ERK (pT185/pY187) links TCR activation to gene expression and cell proliferation.
Preparation Note
Recommended storage for kit components is 2 - 8°C
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
Features and Benefits
- Simultaneously detect 7 phosphorylated proteins involved in T-cell receptor signaling
- Includes CD3ζ, LAT, Lck, ZAP70, PLCγ1, SLP-76, and ERK for comprehensive immune profiling
- Magnetic bead-based format ensures reproducibility and efficient processing
- Compatible with cell lysates from T-cell activation and immunotherapy studies
- Ideal for adaptive immunity, immune modulation, and cell signaling research
- Fast, convenient alternative to Western Blotting and immunoprecipitation procedures
signalword
Danger
Storage Class
10 - Combustible liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
Regulatory Information
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Related Content
Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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