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48-669MAG

Millipore

MILLIPLEX® Early Apoptosis Magnetic Bead 7-plex Kit - Cell Signaling Multiplex Assay

Synonym(s):

Cell Signaling Magnetic Beads, Cell Signaling Multiplex Kit, Total/Phospho Erk Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47

species reactivity

human

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex® xMAP®)

storage temp.

2-8°C

Related Categories

General description

Apoptosis is an essential physiological process that plays a crucial role in normal development and homeostatic mechanisms of multicellular organisms. At least two major apoptotic pathways have been described in mammalian cells: the intrinsic pathway and the extrinsic pathway. Bcl-2 family members also play a pivotal role in the regulation of apoptosis as they reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria.The MILLIPLEX® 7-plex Early Apoptosis Magnetic Bead Kit (Cat. No. 48-669MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes:Akt/PKB (Ser473)BAD (Ser112)Bcl-2 (Ser70)Active Caspase-8Active Caspase-9JNK/SAPK1 (Thr183/Tyr185)p53 (Ser46)

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 2 (provided).The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:Akt (pS473);BAD (Ser112);Bcl-2 (Ser70);p53 (Ser46);JNK (Thr183/Tyr185);Active Caspase-8 (Asp384);Active Caspase-9 (Asp315)

Packaging

96-well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

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