MILLIPLEX^® TGFβ Signaling Pathway Panel

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

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For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Cells respond to their environment in many different ways through intracellular signaling. The TGFβ pathway plays a central role in a number of normal cellular processes including proliferation, differentiation, apoptosis, migration, adhesion, immune response and other functions in most cells. Although the TGFβ pathway regulates a wide range of processes, the pathway is fairly simple. TGFβ dimers bind to TGFβ Type II Receptor which recruits and phosphorylates TGFβ Type I Receptor. The Type I Receptor then recruits and phosphorylates SMAD2/3, which then binds to SMAD4 and forms a complex that enters the cell nucleus where it acts as a transcription factor for various genes. TGFβ Receptor activates the SMAD-dependent canonical pathway, as well as SMAD-independent non-canonical pathways, such as PI3K/Akt, ERK, JNK, and p38. Aberrant TGFβ signaling is involved in the development of multiple diseases, including hematological malignancies such as leukemia, and impaired wound healing, neurodegenerative diseases, developmental disorders and pulmonary hypertension. Loss-of-function mutations promote tumorigenesis by suppressing the immune system and the epithelial mesenchymal transition (EMT), although TGFβ signaling has also been implicated in tumor inhibition. Immune cells such as B cells, T cells, dendritic cells and macrophages secrete TGFβ, which downregulates their proliferation, differentiation and activation by other cytokines. Consequently, modifications in TGFβ signaling have been linked to autoimmune and inflammatory diseases and cancer.

This panel targets phosphorylated proteins in the TGFβ signaling pathway, which regulates cell growth, differentiation, and immune responses. SMAD2 (pS465/pS467) and SMAD3 (pS423/pS425) are direct mediators of TGFβ receptor activation. TAK1 (pS412), p38 (pT180/pY182), ERK1/2 (pT185/pY187), and JNK (pT183/pY185) represent downstream MAPKs involved in inflammation, fibrosis, and cancer progression.

96-well plate

Recommended storage for kit components is 2 - 8°C.

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in cell lysate samples.Analytes Included: SMAD2 (Phospho-Ser465/467), SMAD3 (Phospho-Ser423/425), TAK1 (Phospho-Ser412), p38 (Phospho-Thr180/Tyr182), ERK1/2 (Phospho-Thr185/Tyr187), JNK (Phospho-Thr183/Tyr185)Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).