480401-M
NFAT Inhibitor, Cell-Permeable
The NFAT Inhibitor, Cell-Permeable controls the biological activity of NFAT. This small molecule/inhibitor is primarily used for Inflammation/Immunology applications.
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Synonym(s):
NFAT Inhibitor, Cell-Permeable, 11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG- MAGPHPVIVITGPHEE, 11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG-MAGPHPVIVITGPHEE
Empirical Formula (Hill Notation):
C147H259N67O36S
Molecular Weight:
3573.12
UNSPSC Code:
12352202
NACRES:
NA.77
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Quality Level
Assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
white
solubility
water: 1 mg/mL
DMSO: 5 mg/mL
storage temp.
−20°C
General description
A cell-permeable version of NFAT Inhibitor (Cat. No. 480402) that is modified at the N-terminal with an eleven arginine transduction domain and a three glycine linker sequence. Prevents the activation and proliferation of T cells both in vitro ( ~ 43% at 1 µM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). Shown to be effective in providing immunosuppression in a murine islet xenograft model. Unlike Cyclosporin A (Cat. No. 239835) and FK506, 11R-VIVIT does not cause side effects outside the immune system as a result of calcineurin (Cat. No. 539568) inhibition and therefore serves as a less toxic alternative for in vivo applications. The functional half-life of 11R-VIVIT has been estimated to be ~30 h in Jurkat cells.
A cell-permeable version of NFAT Inhibitor (Cat. No. 480402) that is modified at the C-terminus with an 11-arginine transduction domain and a 3-glycine linker sequence. Prevents the activation and proliferation of T cells both in vitro (~43% at 1 µM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). Show to be effective in providing immunosuppression in a murine islet xenograft model. Unlike Cyclosporin A (Cat. No. 239835) and FK-506, 11R-VIVIT does not cause side effects outside the immune system as a result of calcineurin (Cat. No. 539568) inhibition, therefore serves as a less toxic alternative for animal studies. The functional half-life of 11R-VIVIT has been estimated to be ~30 h in Jurkat cells.
Application
Use at 1 µM for preventing the activation and proliferation of T cells
Biochem/physiol Actions
Primary Target
NFAT
NFAT
Warning
Toxicity: Harmful (C)
Sequence
H-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Gly-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Noguchi, H., et al. 2004. Nat. Med.10, 305.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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