475904-M
MOWIOL® 4-88 Reagent
Synonym(s):
Poly(vinyl alcohol)
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About This Item
Linear Formula:
[-CH2CHOH-]n
CAS Number:
NACRES:
NA.75
UNSPSC Code:
41202101
MDL number:
InChI
1S/C2H4O/c1-2-3/h2-3H,1H2
InChI key
IMROMDMJAWUWLK-UHFFFAOYSA-N
description
RTECS - TR8100000
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
yellow
storage temp.
10-30°C
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Disclaimer
Toxicity: Standard Handling (A)
General description
Used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.
Other Notes
Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell18, 375.
Beug, H., et al. 1979. Cell18, 375.
Preparation Note
Add 2.4 g of Mowiol® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the Mowiol 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Mowiol is a registered trademark of Kuraray Europe GmbH
Storage Class
11 - Combustible Solids
wgk
WGK 1
Regulatory Information
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