475901
Monocyte Chemotactic Protein-1, Human, Recombinant, E. coli
A potent chemoattractant for human monocytes and mouse peritoneal macrophages. Its effects are proposed to be mediated by P2-integrins.
Synonym(s):
rhMCP-1
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About This Item
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Quality Level
Assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotoxins (EU/μg MCP-1)
shipped in
ambient
storage temp.
−70°C
General description
Recombinant, human monocyte chemotactic protein-1 expressed in E. coli. A potent chemoattractant for human monocytes and mouse peritoneal macrophages. Its effects are proposed to be mediated by P2-integrins.
Biochem/physiol Actions
ED₅₀ = 5-20 ng/ml as measured by its monocyte chemotactic activity
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg MCP-1.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
Other Notes
Hosang, K., et al. 1994. Biochem. Biophys. Res. Commun.199, 962.
Luini, W., et al. 1994. Cytokine 6, 28.
Luini, W., et al. 1994. Cytokine 6, 28.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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