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Merck
CN

444258

MMP-14 Substrate I

≥97% (HPLC), Flourescent MMP Substrate, lyophilized solid

Synonym(s):

MMP-14 Substrate I, Fluorogenic, MT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂

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About This Item

Empirical Formula (Hill Notation):
C66H82N16O17S
Molecular Weight:
1403.52
UNSPSC Code:
12352200
NACRES:
NA.77

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Product Name

MMP-14 Substrate I, Fluorogenic, A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity.

assay

≥97% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 5 mg/mL

fluorescence

λex ~328 nm
λem ~400 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
MMP-14
Product does not compete with ATP.
Reversible: no
kcat/Km = 7.3 x 105 M-1s-1 for MMP-14; .6 x 104 M-1s-1 for MMP-11

Disclaimer

Toxicity: Standard Handling (A)

General description

A fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (kcat/km = 730,000 M-1s-1 for MMP-14 (MT1-MMP) and 36,000 M-1s-1 for MMP-11 (ST3); pH = 7.5 at 25°C). Excitation max.: ~328 nm; emission max.: ~400 nm.
A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. (kcat/Km = 7.3 x 105 M-1s-1 for MMP-14 (MT1-MMP) and 3.6 x 104 M-1s-1 for MMP-11 at 25°C, pH 7.5).

Other Notes

MCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl
Mucha, A., et al. 1998. J. Biol. Chem.273, 2763.

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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