444256
MMP Substrate III, Fluorogenic
A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.
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Synonym(s):
MMP Substrate III, Fluorogenic, DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
Empirical Formula (Hill Notation):
C63H88N16O14S
Molecular Weight:
1325.54
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
Quality Level
Assay
≥97% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
red
solubility
DMSO: soluble
Tris-buffered saline, pH 7.6: soluble
fluorescence
λex ~340 nm
λem ~485 nm
shipped in
ambient
storage temp.
−20°C
General description
A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, 21,000 M-1s-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.
A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, and 21,000 M-1s-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Detection of MMP activity
Detection of MMP activity
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Sequence
DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Beekman, B., et al. 1996. FEBS Lett390, 221.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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