Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

444203

Millipore

β-Mercaptoethanol, Molecular Biology Grade

A disulfide-reducing agent.

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
2-Mercaptoethanol, BME, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, Thioethylene glycol
Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
Beilstein:
773648
MDL number:
MFCD00004890
UNSPSC Code:
12161504
NACRES:
NA.25

grade

Molecular Biology

Quality Level

100

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

Assay

≥99% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

mp

<-50 °C

density

1.12 g/cm3 at 20 °C
1.114 g/mL at 25 °C (lit.)

cation traces

heavy metals (as Pb): ≤0.0001%

absorption

≤0.31 at 280 nm
≤1.50 at 260 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

A disulfide-reducing agent.
A disulfide-reducing agent. Reagent commonly used in organic synthesis (aborsorbance: ≤1.50 at 260 nm; ≤0.31 at 280 nm).

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Warning

Toxicity: Toxic (F)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Target Organs

Liver,Heart

WGK

WGK 3

Flash Point(F)

165.2 °F

Flash Point(C)

74 °C

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service