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Merck
CN

437627

Lipopolysaccharide, E. coli O111: B4

from E. coli, , lyophilized, iNOS stimulator, Calbiochem®

Synonym(s):

E. coli Lipopolysaccharide, E. coli O111:B4 LPS

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.77

Key Documents

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Product Name

Lipopolysaccharide, E. coli O111: B4, Lipopolysaccharide, E. coli O111: B4 stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes

description

RTECS - OJ0895400

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤2.0% Protein
≤2.5% nucleic acid

solubility

soluble (H2O (5 mg/ml) or 0.5% triethylamine (1 mg/ml). To achieve complete solubilization, heating solution to 37°C with intermittent vortexing or sonication may be necessary.)

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
Inducible nitric oxide synthase
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Harmful (C)

General description

Endotoxin lipopolysaccharides initiate a variety of biochemical cell activation pathways (PKC, cAMP-dependent protein kinase, arachidonate metabolism, protein myristoylation, and activation of G-proteins). These unique macromolecules have been extensively studied in order to elucidate and define relevant pathophysiological parameters of endotoxic shock, a profound life-threatening consequence of sepsis. More recently, these bacterial products have generated intense interest due to their pluropotential immunostimulatory activity as manifested by the activation of host cells (e.g. B lymphocytes, macrophages) to functional differentiation. Host cell activation by lipopolysaccharides produces a spectrum of hormone-active lymphokines and monokines, including interferons (α, β, and γ), interleukins (IL-1 and IL-6), tumor necrosis factor, platelet activating factor, and procoagulant tissue factor. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.
Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Other Notes

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int. 35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol. 49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem. 60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.
Jackson, S.K., et al. 1992. Biochim. Biophys. Acta1135, 165.
O′Sullivan, M.G., et al. 1992. J. Biol. Chem.267, 14547.
Watanabe, K., et al. 1992. J. Cell. Physiol.150, 433.
Morrison, D.C., and Ryan, J.L. 1987. Annu. Rev. Med.38, 417.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
To achieve complete solubilization, heating solution to 37°C with intermittent vortexing or sonication may be necessary.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhan Zhao et al.
Journal of neuroinflammation, 17(1), 64-64 (2020-02-20)
Sepsis-associated acute brain inflammation, if unresolved, may cause chronic neuroinflammation and resultant neurodegenerative diseases. However, little is known how the transition from acute to chronic neuroinflammation, which is critical for the following progressive neurodegeneration, occurs in sepsis. The goal of

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