Skip to Content
Merck
CN
All Photos(1)

Documents

431525

Sigma-Aldrich

L-Leucine Dehydrogenase, Bacillus cereus

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD +-oxidoreductase
CAS Number:
9082-71-7
Enzyme Commission number:
1.4.1.9 (BRENDA, IUBMB)
MDL number:
MFCD00147461
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

Bacillus sp. (Bacillus cereus)

Quality Level

100

form

liquid

specific activity

≥10 units/mg protein
≥50 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

General description

Native L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD+-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.
Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.
pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD+ per min at 30°C, pH 10.7.

Physical form

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service