420291
IPTG, Animal-Free, High Purity
Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.
Synonym(s):
IPTG, Animal-Free, High Purity, Isopropyl-β-D-thiogalactopyranoside
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About This Item
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.25
Quality Level
Assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
impurities
Dioxane, none detected
color
white to off-white
solubility
water: 100 mg/mL
methanol: soluble
shipped in
ambient
storage temp.
2-8°C
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
Related Categories
General description
Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.
Application
- Mobile-CRISPRi as a powerful tool for modulating Vibrio gene expression.: This article explores the use of Mobile-CRISPRi for targeted gene regulation in Vibrio species, highlighting potential applications in microbial genetics and biotechnological innovations, including the manipulation of gene expression pathways facilitated by IPTG-induced systems (Geyman et al., 2024).
- A new Zymomonas mobilis platform strain for the efficient production of chemicals.: This research describes the development of a Zymomonas mobilis strain engineered for enhanced chemical production, utilizing IPTG for controlled expression of metabolic pathways, showcasing significant implications for biofuel production and industrial biotechnology (Frohwitter et al., 2024).
- Biophysical characterization and insights into the oligomeric nature of CD2-associated protein.: The study provides a detailed biophysical analysis of CD2-associated protein, employing IPTG-induced expression to elucidate its oligomeric structure and function in cellular signaling, offering insights into therapeutic target development (Qadri et al., 2024).
- Effective delivery of anti-PD-L1 siRNA with human heavy chain ferritin (HFn) in acute myeloid leukemia cell lines.: This research utilizes IPTG-inducible systems to study the delivery of therapeutic siRNAs in leukemia treatment, demonstrating the potential of IPTG in enhancing the efficacy of gene therapy approaches in oncology (Rajabinejad et al., 2024).
- Heterologous expression of nattokinase in E. coli: Biochemical characterization and functional analysis of fibrin binding residues.: This article details the heterologous expression of nattokinase in E. coli, facilitated by IPTG induction, focusing on its potential applications in thrombolytic therapy and its biochemical properties relevant to medical biochemistry (Jain et al., 2024).
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Lama, J., et al. 1992. Gene117, 185.
Zhang, Y., and Moss, B. 1991. Natl. Acad. Sci. USA88, 1511.
Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347.
Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598.
Lee, S.G., et al. 1988. Biochemistry27, 2983.
Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268.
Zhang, Y., and Moss, B. 1991. Natl. Acad. Sci. USA88, 1511.
Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347.
Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598.
Lee, S.G., et al. 1988. Biochemistry27, 2983.
Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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