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Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069)

liquid, clone HP6069, Calbiochem®

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UNSPSC Code:
12352203
NACRES:
NA.74

biological source

mouse

Quality Level

200

antibody form

purified antibody

antibody product type

secondary antibodies

clone

HP6069, monoclonal

form

liquid

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Purified mouse monoclonal antibody. Recognizes human IgG1 Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) is validated for use in ELISA for the detection of Human IgG₁, Fc Fragment Specific.

Application

ELISA (see comments and application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use aliquot and freeze (-20°C).

Other Notes

Can be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: 100%

Human IgG2: 0.1%

Human IgG3: 0.1%

Human IgG4: <0.02%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.01%

Human IgG2: <0.01%

Human IgG3: <0.01%

Human IgG4: <0.01%

Human IgA: <0.01%

Human IgE: <0.001%

Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elizabeth H Aitken et al.
eLife, 10 (2021-06-29)
Plasmodium falciparum causes placental malaria, which results in adverse outcomes for mother and child. P. falciparum-infected erythrocytes that express the parasite protein VAR2CSA on their surface can bind to placental chondroitin sulfate A. It has been hypothesized that naturally acquired
D C Houpt et al.
Infection and immunity, 62(7), 2857-2864 (1994-07-01)
Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
R G Keller et al.
Infection and immunity, 62(1), 215-220 (1994-01-01)
Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as
H Lu et al.
Infection and immunity, 61(6), 2400-2407 (1993-06-01)
The aims of this study were to determine the immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b (Aab) for the different immunoglobulin (Ig) classes and subclasses and to determine the relative levels of these different Igs in serum. Seropositive early-onset periodontitis

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