393206
Anti-HNE Fluorophore Rabbit pAb
liquid, Calbiochem®
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Synonym(s):
Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
diluted serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
General description
Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
Immunogen
a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
Application
ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Warning
Toxicity: Standard Handling (A)
Physical form
Serum diluted 1:1 with glycerol.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Certificates of Analysis (COA)
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