374807
Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer
Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ buffer, CAS 9013-72-3, is a large, multi-subunit, O2-carrying, metalloprotein that is used in the production of antibodies.
Synonym(s):
Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer, KLH
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About This Item
Quality Level
Assay
≥90% (size exclusion chromatography)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
color
clear blue-gray
shipped in
wet ice
storage temp.
2-8°C
General description
Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. It is isolated from a marine snail species called Megathura crenulate.
Biochem/physiol Actions
Keyhole limpet hemocyanin (KLH) exhibits immunostimulatory properties and stimulates both cell-mediated and humoral responses in animals and humans. It serves as a vaccine adjuvant and facilitates protein conjugation. In addition, KLH acts as a carrier protein to produce antibodies as well as haptens such as peptides and oligosaccharides. It is also used in the treatment of bladder cancer.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 10 mM MgSO₄, 5 mM BES buffer, 0.9% NaCl, pH 6.5. Sterile-filtered.
Preparation Note
KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it
Evaluation of human antibody responses to keyhole limpet hemocyanin on a carbohydrate microarray
Oyelaran O and Gildersleeve JC, et al.
4th International Conference on Biomedical Engineering in Vietnam, 285-294 (2010)
Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
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