362280
Glycoprotein Deglycosylation Kit
Synonym(s):
Deglycosylation kit
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About This Item
UNSPSC Code:
41116133
NACRES:
NA.84
General description
Kit contains all enzymes and reagents needed to remove all N-linked, all simple O-linked, and virtually all-complex O-linked oligosaccharides from glycoproteins in a single reaction at neutral pH. Suitable for use with native or denaturation protocols, with no degradation of protein. This kit has been assembled to contain sequencing grade enzymes without azide, BSA, or glycerol. Each kit can be used to deglycosylate up to 2 mg glycoprotein.
Components
Bovine Fetuin Control, Denaturation Solution, Endo-α-N-Acetylgalactosaminidase, β1,4-Galactosidase, Glucosaminidase, N-Glycosidase F, Neuraminidase, Reaction Buffer, TRITON™ X-100 Detergent, and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Specifications
Assay Time: 3 h-5 days
Other Notes
Chiba, A., et al. 1997. J. Biol. Chem.272, 2156.
Jiang, M.S., and Hart, G.W. 1997. J. Biol. Chem.272, 2421.
Wilkins, P.P., et al. 1996. J. Biol. Chem.271, 18732.
Altmann, F., et al. 1995. Glycoconj. J.12, 84.
Szkudlinski, M.W., et al. 1995.
Endocrinology136, 3325.
Pahlsson, P., et al. 1994. Glycoconj. J.11, 43.
Saito, S., et al. 1994. J. Biol. Chem.269, 5644.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol.230, 44.
Iwase, H., Hotta, K. 1993. Methods. Mol. Biol.14, 151.
Stults, N.L., and Cummings, R.D. 1993. Glycobiology3, 589.
Hard, K., et al. 1992. Eur. J. Biochem.205, 785.
Kuraya, N., and Hase, S. 1992. J. Biochem. (Tokyo)112, 122.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem.266, 1646.
Fukuda, M., et al. 1987. J. Biol. Chem.262, 11952.
Sojar, H.T., and Bahl, O.P. 1987. Arch. Biochem. Biophys.259, 52.
Taga, E.M., et al. 1984. Biochemistry23, 815.
Kobata, A. 1979. Anal. Biochem.100, 1.
Uchida, Y., et al. 1979. J. Biochem (Tokyo)86, 1573.
Glasgow, L.R., et al. 1977. J. Biol. Chem.252, 8615.
Spiro, R.G., and Bhoyroo, V.D. 1974. J. Biol. Chem.249, 5704.
Jiang, M.S., and Hart, G.W. 1997. J. Biol. Chem.272, 2421.
Wilkins, P.P., et al. 1996. J. Biol. Chem.271, 18732.
Altmann, F., et al. 1995. Glycoconj. J.12, 84.
Szkudlinski, M.W., et al. 1995.
Endocrinology136, 3325.
Pahlsson, P., et al. 1994. Glycoconj. J.11, 43.
Saito, S., et al. 1994. J. Biol. Chem.269, 5644.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol.230, 44.
Iwase, H., Hotta, K. 1993. Methods. Mol. Biol.14, 151.
Stults, N.L., and Cummings, R.D. 1993. Glycobiology3, 589.
Hard, K., et al. 1992. Eur. J. Biochem.205, 785.
Kuraya, N., and Hase, S. 1992. J. Biochem. (Tokyo)112, 122.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem.266, 1646.
Fukuda, M., et al. 1987. J. Biol. Chem.262, 11952.
Sojar, H.T., and Bahl, O.P. 1987. Arch. Biochem. Biophys.259, 52.
Taga, E.M., et al. 1984. Biochemistry23, 815.
Kobata, A. 1979. Anal. Biochem.100, 1.
Uchida, Y., et al. 1979. J. Biochem (Tokyo)86, 1573.
Glasgow, L.R., et al. 1977. J. Biol. Chem.252, 8615.
Spiro, R.G., and Bhoyroo, V.D. 1974. J. Biol. Chem.249, 5704.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Regulatory Information
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