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Sigma-Aldrich

TWS119

≥95% (HPLC), solid, GSK-3β inhibitor, Calbiochem®

Synonym(s):

GSK-3β Inhibitor XII, TWS119, Neurogenesis Inducer, TWS119

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About This Item

Empirical Formula (Hill Notation):
C18H14N4O2
CAS Number:
601514-19-6
Molecular Weight:
318.33
MDL number:
MFCD09037535
UNSPSC Code:
12352200
NACRES:
NA.77

product name

GSK-3β Inhibitor XII, TWS119, The GSK-3β Inhibitor XII, TWS119, also referenced under CAS 601514-19-6, controls the biological activity of GSK-3β. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Quality Level

100

Assay

≥95% (HPLC)

form

solid

potency

30 nM IC50

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

beige

solubility

methanol: 2 mg/mL
DMSO: 200 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C18H14N4O2/c19-12-4-1-3-11(7-12)16-9-15-17(22-16)20-10-21-18(15)24-14-6-2-5-13(23)8-14/h1-10,23H,19H2,(H,20,21,22)

InChI key

VPVLEBIVXZSOMQ-UHFFFAOYSA-N

General description

A cell-permeable and potent inhibitor of GSK-3β (IC50 = 30 nM). Binds to GSK-3β with high-affinity (Kd = 126 nM), leading to an increase in the level of β-catenin, a downstream substrate of GSK-3β in the Wnt signaling pathway. Shown to selectively induce neuronal differentiation in both pluripotent murine embryonal carcinoma cells (P19; 30-40% at 1 µM) and embryonic stem cells (D3; 50-60% at 400 nM).
A cell-permeable pyrrolopyrimidine compound that acts as a potent inhibitor of GSK-3β (IC50 = 30 nM). Binds to GSK-3β with high-affinity (Kd = 126 nM) and increases the level of β-catenin, a downstream substrate of GSK-3β in the Wnt signaling pathway. Shown to selectively induce neuronal differentiation in both pluripotent murine embryonal carcinoma cells (P19; 30-40% at 1 µM) and embryonic stem cells (D3; 50-60% at 400 nM).

Biochem/physiol Actions

Cell permeable: yes
Kd = 126 nM against GSK-3β
Primary Target
GSK-3β
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ding, S., et al. 2003. Proc. Natl. Acad. Sci. USA100, 7632.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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