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Merck
CN

345852

Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

Geranylgeranyltransferase I, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.

Synonym(s):

GGTase I

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352202

Key Documents

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Product Name

Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli, Geranylgeranyltransferase I, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Biochem/physiol Actions

1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn2+ for optimal activity. The enzyme is purified with Zn2+ tightly bound, so addition of Zn2+ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.

Other Notes

Kalinin, A., et al. 2001. Protein Expr. Purif.22, 84.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.

Physical form

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Shahar Molshanski-Mor et al.
Methods in molecular biology (Clifton, N.J.), 412, 385-428 (2008-05-06)
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