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Merck
CN

345806-M

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

Synonym(s):

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli, β1,4-D-Galactoside Galactohydrolase

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About This Item

CAS Number:
EC Number:
NACRES:
NA.77
UNSPSC Code:
12352202
MDL number:
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recombinant

expressed in E. coli

form

liquid

specific activity

≥1.5 units/mL
≥6 units/mg protein

packaging

pkg of 50 mIU

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected

storage temp.

2-8°C

Quality Level

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Disclaimer

Toxicity: Standard Handling (A)

General description

Note: 1 mU = 1 milliunit.
Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Other Notes

Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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