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About This Item
CAS Number:
NACRES:
NA.77
UNSPSC Code:
12352202
MDL number:
recombinant
expressed in E. coli
form
liquid
specific activity
≥1.5 units/mL
≥6 units/mg protein
packaging
pkg of 50 mIU
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
storage temp.
2-8°C
Quality Level
Application
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
Disclaimer
Toxicity: Standard Handling (A)
General description
Note: 1 mU = 1 milliunit.
Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Other Notes
Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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